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Minimum primer Tm Maximum primer GC% Minimum primer GC% Maximum complementarity Maximum 3' complementarity Salt concentration (mM) Annealing primer concentration (nM) Number of outputs Help For any questions or comments, please contact [email protected] References: Andrew Collins, Xiayi Ke Primer1: primer design web service for tetra-primer ARMS-PCR. blast primer,The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between ... Primer-BLAST. Design primers specific to your PCR template.Primer Designing Using NCBI Primer Blast. SciArtFun Education. How To Design primers Using NCBI Primer Blast. Search www.ncbi.nlm.nih.gov then select gene ...